4ESJ

RESTRICTION ENDONUCLEASE DpnI IN COMPLEX WITH TARGET DNA

Summary for 4ESJ

DescriptorType-2 restriction enzyme DpnI, DNA (5'-D(*CP*TP*GP*GP*(6MA)P*TP*CP*CP*AP*G)-3'), ZINC ION, ... (7 entities in total)
Functional Keywordsrestriction endonuclease-dna complex, type iim, type iie, restriction enzyme, dpni, methylation dependent, n6-methyladenine, pd-(d/e)xk type endonuclease, winged helix domain, restriction endonuclease, dna binding, hydrolase-dna complex, hydrolase/dna
Biological sourceStreptococcus pneumoniae
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Total number of polymer chains6
Total molecular weight72723.18
Authors
Siwek, W.,Czapinska, H.,Bochtler, M.,Bujnicki, J.M.,Skowronek, K. (deposition date: 2012-04-23, release date: 2012-06-13, Last modification date: 2012-10-17)
Primary citation
Siwek, W.,Czapinska, H.,Bochtler, M.,Bujnicki, J.M.,Skowronek, K.
Crystal structure and mechanism of action of the N6-methyladenine-dependent type IIM restriction endonuclease R.DpnI.
Nucleic Acids Res., 40:7563-7572, 2012
PubMed: 22610857 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gks428
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.05 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.218300.2%10.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution