4ESJ
RESTRICTION ENDONUCLEASE DpnI IN COMPLEX WITH TARGET DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-05 |
| Detector | ADSC Q315 |
| Wavelength(s) | 0.97949,1.2833,1.2835 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.518, 98.411, 83.884 |
| Unit cell angles | 90.00, 112.21, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.050 |
| R-factor | 0.19872 |
| Rwork | 0.198 |
| R-free | 0.21754 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.028 | 0.300 |
| Number of reflections | 60190 | |
| <I/σ(I)> | 17.5 | 2.4 |
| Completeness [%] | 98.0 | 98.4 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 200 MM POTASSIUM SULFATE, 100 MM BETAINE, 20 % W/V PEG 3350, PH 6.8. FOR CRYOCOOLING THE CRYSTALLIZATION BUFFER WAS MIXED IN 3:1 RATIO WITH 100% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
