4EMU

Crystal structure of ligand free human STING

Summary for 4EMU

• Entry DOI: 10.2210/pdb4emu/pdb
• Related: 4EMT
• Descriptor: Transmembrane protein 173, CALCIUM ION (3 entities in total)
• Functional Keywords: alpha/beta fold, innate immune sensor, c-di-gmp, membrane protein
• Biological source: Homo sapiens (human)
• Cellular location: Endoplasmic reticulum membrane ; Multi-pass membrane protein : Q86WV6
• Total number of polymer chains: 2
• Total formula weight: 43088.39

Authors

Li, P. (deposition date: 2012-04-12, release date: 2012-06-13, Last modification date: 2024-02-28)

Primary citation

Shu, C.,Yi, G.,Watts, T.,Kao, C.C.,Li, P.
Structure of STING bound to cyclic di-GMP reveals the mechanism of cyclic dinucleotide recognition by the immune system.
Nat.Struct.Mol.Biol., 19:722-724, 2012

PubMed Abstract: STING (stimulator of interferon genes) is an innate immune sensor of cyclic dinucleotides that regulates the induction of type I interferons. STING's C-terminal domain forms a V-shaped dimer and binds a cyclic diguanylate monophosphate (c-di-GMP) at the dimer interface by both direct and solvent-mediated hydrogen bonds. Guanines of c-di-GMP stack against the phenolic rings of a conserved tyrosine, and mutations at the c-di-GMP binding surface reduce nucleotide binding and affect signaling.

PubMed: 22728658
DOI: 10.1038/nsmb.2331

Experimental method

X-RAY DIFFRACTION (1.9 Å)