4EC3

Structure of berberine bridge enzyme, H174A variant in complex with (S)-reticuline

Summary for 4EC3

• Entry DOI: 10.2210/pdb4ec3/pdb
• Related: 3D2D 3D2J 3FW7 3FW8
• Descriptor: Reticuline oxidase, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, FLAVIN-ADENINE DINUCLEOTIDE, ... (7 entities in total)
• Functional Keywords: p-cresol methyl hydroxylase superfamily, oxidoreductase, fad, bi-covalent flavinylation
• Biological source: Eschscholzia californica (California poppy)
• Cellular location: Cytoplasmic vesicle: P30986
• Total number of polymer chains: 1
• Total formula weight: 60252.82

Authors

Winkler, A.,Macheroux, P.,Gruber, K. (deposition date: 2012-03-26, release date: 2012-07-18, Last modification date: 2023-09-13)

• Primary citation: Wallner, S.,Winkler, A.,Riedl, S.,Dully, C.,Horvath, S.,Gruber, K.,Macheroux, P.; Catalytic and structural role of a conserved active site histidine in berberine bridge enzyme.; Biochemistry, 51:6139-6147, 2012; PubMed: 22757961; DOI: 10.1021/bi300411n

Experimental method

X-RAY DIFFRACTION (2.6501 Å)