4E8R
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of Cs+ and Mg2+
Summary for 4E8R
| Entry DOI | 10.2210/pdb4e8r/pdb |
| Related | 4E8K 4E8M 4E8N 4E8P 4E8Q 4E8T 4E8V |
| Descriptor | Group IIC intron, MAGNESIUM ION, CESIUM ION, ... (5 entities in total) |
| Functional Keywords | ribozyme, metalloenzyme, self-splicing, retrotransposition, rna |
| Biological source | Oceanobacillus iheyensis |
| Total number of polymer chains | 1 |
| Total formula weight | 134048.80 |
| Authors | Marcia, M.,Pyle, A.M. (deposition date: 2012-03-20, release date: 2012-11-14, Last modification date: 2023-09-13) |
| Primary citation | Marcia, M.,Pyle, A.M. Visualizing Group II Intron Catalysis through the Stages of Splicing. Cell(Cambridge,Mass.), 151:497-507, 2012 Cited by PubMed Abstract: Group II introns are self-splicing ribozymes that share a reaction mechanism and a common ancestor with the eukaryotic spliceosome, thereby providing a model system for understanding the chemistry of pre-mRNA splicing. Here we report 14 crystal structures of a group II intron at different stages of catalysis. We provide a detailed mechanism for the first step of splicing, we describe a reversible conformational change between the first and the second steps of splicing, and we present the ligand-free intron structure after splicing in an active state that corresponds to the retrotransposable form of the intron. During each reaction, the reactants are aligned and activated by a heteronuclear four-metal-ion center that contains a metal cluster and obligate monovalent cations, and they adopt a structural arrangement similar to that of protein endonucleases. Based on our data, we propose a model for the splicing cycle and show that it is applicable to the eukaryotic spliceosome. PubMed: 23101623DOI: 10.1016/j.cell.2012.09.033 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.36 Å) |
Structure validation
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