4E68

Unphosphorylated STAT3B core protein binding to dsDNA

Summary for 4E68

• Entry DOI: 10.2210/pdb4e68/pdb
• Related: 1BG1
• Descriptor: DNA (5'-D(*TP*GP*CP*AP*TP*TP*TP*CP*CP*CP*GP*TP*AP*AP*AP*TP*CP*T)-3'), Signal transducer and activator of transcription 3 (3 entities in total)
• Functional Keywords: sh2 domain, protein dna complex, signal transducer, activator of transcription, duplex dna, nucleus, cytosol, transcription-dna complex, transcription/dna
• Biological source: Mus musculus (mouse); More
• Cellular location: Cytoplasm: 4E68
• Total number of polymer chains: 2
• Total formula weight: 73576.66

Authors

Collie, G.W.,Parkinson, G.N.,Shah, R. (deposition date: 2012-03-15, release date: 2013-03-06, Last modification date: 2023-09-13)

Primary citation

Nkansah, E.,Shah, R.,Collie, G.W.,Parkinson, G.N.,Palmer, J.,Rahman, K.M.,Bui, T.T.,Drake, A.F.,Husby, J.,Neidle, S.,Zinzalla, G.,Thurston, D.E.,Wilderspin, A.F.
Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography.
Febs Lett., 587:833-839, 2013

PubMed Abstract: The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705.

PubMed: 23434585
DOI: 10.1016/j.febslet.2013.01.065

Experimental method

X-RAY DIFFRACTION (2.585 Å)