4E68
Unphosphorylated STAT3B core protein binding to dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91730 |
Spacegroup name | I 41 |
Unit cell lengths | 174.140, 174.140, 79.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 2.585 |
R-factor | 0.22778 |
Rwork | 0.225 |
R-free | 0.27332 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bg1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.732 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.160 | 2.650 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.056 | 0.527 |
Number of reflections | 36458 | |
<I/σ(I)> | 15.2 | 2.3 |
Completeness [%] | 97.6 | 99.3 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.005M MgSO4, 0.1M ammonium acetate, 10% glycerol, 0.05M MES pH 5.6, 0.25M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |