4DOU

Crystal Structure of a Single-chain Trimer of Human Adiponectin Globular Domain

Summary for 4DOU

• Entry DOI: 10.2210/pdb4dou/pdb
• Related: 1C28
• Descriptor: Adiponectin, CALCIUM ION, SODIUM ION, ... (6 entities in total)
• Functional Keywords: single-chain globular domain, calcium binding, c1q-like domain, growth factor, serum, hormone
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 1
• Total formula weight: 51548.90

Authors

Min, X.,Walker, N.P.,Wang, Z. (deposition date: 2012-02-10, release date: 2012-02-29, Last modification date: 2023-09-13)

Primary citation

Min, X.,Lemon, B.,Tang, J.,Liu, Q.,Zhang, R.,Walker, N.,Li, Y.,Wang, Z.
Crystal structure of a single-chain trimer of human adiponectin globular domain.
Febs Lett., 586:912-917, 2012

PubMed Abstract: Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a single-chain globular domain adiponectin (sc-gAd) in which three monomer sequences are linked together in tandem to form one contiguous polypeptide. Here, we present the crystal structure of human sc-gAd at 2.0Å resolution. The structure reveals a similar trimeric topology to that of mouse gAd protein. Trimer formation is further rigidified by three calcium ions.

PubMed: 22449980
DOI: 10.1016/j.febslet.2012.02.024

Experimental method

X-RAY DIFFRACTION (2 Å)