4DFP

Crystal structure of the large fragment of DNA Polymerase I from Thermus aqauticus in a ternary complex with 7-(aminopentinyl)-7-deaza-dGTP

4DFP の概要

• エントリーDOI: 10.2210/pdb4dfp/pdb
• 関連するPDBエントリー: 3KTQ 3LWL 3LWM 3M8S 3OJS 3OJU
• 分子名称: DNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), 5'-d(AAACGGCGCCGTGGTC)-3', ... (8 entities in total)
• 機能のキーワード: dna polymerase, transferase-dna complex, transferase/dna
• 由来する生物種: Thermus aquaticus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70819.88

構造登録者

Bergen, K.,Steck, A.,Struett, S.,Baccaro, A.,Welte, W.,Diederichs, K.,Marx, A. (登録日: 2012-01-24, 公開日: 2012-05-16, 最終更新日: 2023-09-13)

主引用文献

Bergen, K.,Steck, A.L.,Strutt, S.,Baccaro, A.,Welte, W.,Diederichs, K.,Marx, A.
Structures of KlenTaq DNA Polymerase Caught While Incorporating C5-Modified Pyrimidine and C7-Modified 7-Deazapurine Nucleoside Triphosphates.
J.Am.Chem.Soc., 134:11840-11843, 2012

PubMed Abstract: The capability of DNA polymerases to accept chemically modified nucleotides is of paramount importance for many biotechnological applications. Although these analogues are widely used, the structural basis for the acceptance of the unnatural nucleotide surrogates has been only sparsely explored. Here we present in total six crystal structures of modified 2'-deoxynucleoside-5'-O-triphosphates (dNTPs) carrying modifications at the C5 positions of pyrimidines or C7 positions of 7-deazapurines in complex with a DNA polymerase and a primer/template complex. The modified dNTPs are in positions poised for catalysis leading to incorporation. These structural data provide insight into the mechanism of incorporation and acceptance of modified dNTPs. Our results open the door for rational design of modified nucleotides, which should offer great opportunities for future applications.

PubMed: 22475415
DOI: 10.1021/ja3017889

実験手法

X-RAY DIFFRACTION (2 Å)