4D4D

The catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with 1,6-ManIFG

Summary for 4D4D

• Entry DOI: 10.2210/pdb4d4d/pdb
• Related: 4D4A 4D4B 4D4C 5AGD
• Descriptor: ALPHA-1,6-MANNANASE, 5-HYDROXYMETHYL-3,4-DIHYDROXYPIPERIDINE, alpha-D-mannopyranose, ... (5 entities in total)
• Functional Keywords: hydrolase, glycoside hydrolase, gh76, cazy, mannan, enzyme-carbohydrate interaction, glycosidase inhibition, transition state
• Biological source: BACILLUS CIRCULANS
• Total number of polymer chains: 2
• Total formula weight: 82578.44

Authors

Thompson, A.J.,Speciale, G.,Iglesias-Fernandez, J.,Hakki, Z.,Belz, T.,Cartmell, A.,Spears, R.J.,Stepper, J.,Gilbert, H.J.,Rovira, C.,Williams, S.J.,Davies, G.J. (deposition date: 2014-10-27, release date: 2015-03-25, Last modification date: 2020-07-29)

• Primary citation: Thompson, A.J.,Speciale, G.,Iglesias-Fernandez, J.,Hakki, Z.,Belz, T.,Cartmell, A.,Spears, R.J.,Chandler, E.,Temple, M.J.,Stepper, J.,Gilbert, H.J.,Rovira, C.,Williams, S.J.,Davies, G.J.; Evidence for a Boat Conformation at the Transition State of Gh76 Alpha-1,6-Mannanases- Key Enzymes in Bacterial and Fungal Mannoprotein Metabolism; Angew.Chem.Int.Ed.Engl., 54:5378-, 2015; PubMed: 25772148; DOI: 10.1002/ANIE.201410502

Experimental method

X-RAY DIFFRACTION (1.4 Å)