4D4D

The catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with 1,6-ManIFG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ALPHA-1,6-MANNANASE	polymer	362	40930.9	2	UniProt (Q9Z4P9) Pfam (PF03663)	BACILLUS CIRCULANS
2	C, E (A, B)	5-HYDROXYMETHYL-3,4-DIHYDROXYPIPERIDINE	non-polymer		147.2	2	Chemie (IFM)
3	D, F (A, B)	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
4	G (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	H, I, J, K (A, B)	water	water		18.0	939	Chemie (HOH)

Sequence modifications

A, B: 35 - 375 (UniProt: Q9Z4P9)

PDB	External Database	Details
Met 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Glu 28	-	expression tag
Val 29	-	expression tag
Leu 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
Pro 34	-	expression tag
Gln 341	Arg 341	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81861.7
Non-Polymers*	Number of molecules	5
	Total formula weight	716.7
All*	Total formula weight	82578.4

*Water molecules are not included.