4CS4

Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP

4CS4 の概要

• エントリーDOI: 10.2210/pdb4cs4/pdb
• 関連するPDBエントリー: 4CS2 4CS3
• 分子名称: PYRROLYSYL-TRNA SYNTHETASE, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: ligase, aminoacyl-trna synthetase, noncanonical amino acids
• 由来する生物種: METHANOSARCINA MAZEI
• 細胞内の位置: Cytoplasm (By similarity): Q8PWY1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33917.88

構造登録者

Schmidt, M.J.,Weber, A.,Pott, M.,Welte, W.,Summerer, D. (登録日: 2014-03-04, 公開日: 2014-04-30, 最終更新日: 2024-05-08)

主引用文献

Schmidt, M.J.,Weber, A.,Pott, M.,Welte, W.,Summerer, D.
Structural Basis of Furan-Amino Acid Recognition by a Polyspecific Aminoacyl-tRNA-Synthetase and its Genetic Encoding in Human Cells.
Chembiochem, 15:1755-, 2014

PubMed Abstract: The site-selective introduction of photo-crosslinking groups into proteins enables the discovery and mapping of weak and/or transient protein interactions with high spatiotemporal resolution, both in vitro and in vivo. We report the genetic encoding of a furan-based, photo-crosslinking amino acid in human cells; it can be activated with red light, thus offering high penetration depths in biological samples. This is achieved by activation of the amino acid and charging to its cognate tRNA by a pyrrolysyl-tRNA-synthetase (PylRS) mutant with broad polyspecificity. To gain insights into the recognition of this amino acid and to provide a rationale for its polyspecificity, we solved three crystal structures of the PylRS mutant: in its apo-form, in complex with adenosine 5'-(β,γ-imido)triphosphate (AMP-PNP) and in complex with the AMP ester of the furan amino acid. These structures provide clues for the observed polyspecificity and represent a promising starting point for the engineering of PylRS mutants with further increased substrate scope.

PubMed: 24737732
DOI: 10.1002/CBIC.201402006

実験手法

X-RAY DIFFRACTION (1.349 Å)