4CS4
Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-03 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.075, 44.533, 64.359 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 43.100 - 1.349 |
| R-factor | 0.1497 |
| Rwork | 0.148 |
| R-free | 0.17560 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.100 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 1.420 |
| Number of reflections | 62528 | |
| <I/σ(I)> | 17.67 | 0.74 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM TRIS PH 7.5, 10 % (W/V) PEG 2000 MONOMETHYL ETHER |
