4CIJ

Structure of Rolling Circle Replication Initiator Protein from Geobacillus stearothermophilus.

Summary for 4CIJ

• Entry DOI: 10.2210/pdb4cij/pdb
• Descriptor: GST REP (2 entities in total)
• Functional Keywords: isomerase, cell cycle, rolling circle replication, antibiotic resistance, type i topoisomerase
• Biological source: GEOBACILLUS STEAROTHERMOPHILUS
• Total number of polymer chains: 4
• Total formula weight: 125512.49

Authors

Carr, S.B.,Phillips, S.E.V.,Thomas, C.D. (deposition date: 2013-12-10, release date: 2014-12-24, Last modification date: 2024-05-08)

Primary citation

Carr, S.B.,Phillips, S.E.,Thomas, C.D.
Structures of Replication Initiation Proteins from Staphylococcal Antibiotic Resistance Plasmids Reveal Protein Asymmetry and Flexibility are Necessary for Replication.
Nucleic Acids Res., 44:2417-, 2016

PubMed Abstract: Antibiotic resistance in pathogenic bacteria is a continual threat to human health, often residing in extrachromosomal plasmid DNA. Plasmids of the pT181 family are widespread and confer various antibiotic resistances to Staphylococcus aureus. They replicate via a rolling circle mechanism that requires a multi-functional, plasmid-encoded replication protein to initiate replication, recruit a helicase to the site of initiation and terminate replication after DNA synthesis is complete. We present the first atomic resolution structures of three such replication proteins that reveal distinct, functionally relevant conformations. The proteins possess a unique active site and have been shown to contain a catalytically essential metal ion that is bound in a manner distinct from that of any other rolling circle replication proteins. These structures are the first examples of the Rep_trans Pfam family providing insights into the replication of numerous antibiotic resistance plasmids from Gram-positive bacteria, Gram-negative phage and the mobilisation of DNA by conjugative transposons.

PubMed: 26792891
DOI: 10.1093/NAR/GKV1539

Experimental method

X-RAY DIFFRACTION (2.3 Å)