4CIJ
Structure of Rolling Circle Replication Initiator Protein from Geobacillus stearothermophilus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-30 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.111, 136.848, 149.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.250 - 2.300 |
R-factor | 0.19503 |
Rwork | 0.193 |
R-free | 0.23457 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 1.543 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.200 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 0.860 |
Number of reflections | 60795 | |
<I/σ(I)> | 16.9 | 2.2 |
Completeness [%] | 99.7 | 99 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M PROLINE, 0.1 M HEPES PH 7.5, 10 % (V/V) PEG 3350 |