4CFI

3D structure of FliC from Burkholderia pseudomallei

Summary for 4CFI

• Entry DOI: 10.2210/pdb4cfi/pdb
• Descriptor: FLAGELLIN (2 entities in total)
• Functional Keywords: structural protein, flagella, epitope, immune response
• Biological source: BURKHOLDERIA PSEUDOMALLEI
• Cellular location: Secreted : P70944
• Total number of polymer chains: 1
• Total formula weight: 25899.21

Authors

Lassaux, P.,Peri, C.,Ferrer-Navarro, M.,Gourlay, L.J.,Conchillo-Sole, O.,Daura, X.,Colombo, G.,Bolognesi, M. (deposition date: 2013-11-18, release date: 2014-12-10, Last modification date: 2023-12-20)

Primary citation

Nithichanon, A.,Rinchai, D.,Gori, A.,Lassaux, P.,Peri, C.,Conchillio-Sole, O.,Ferrer-Navarro, M.,Gourlay, L.J.,Nardini, M.,Vila, J.,Daura, X.,Colombo, G.,Bolognesi, M.,Lertmemonkolchai, G.
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia Pseudomallei Flagellin.
Plos Negl Trop Dis, 9:03917-, 2015

PubMed Abstract: Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.

PubMed: 26222657
DOI: 10.1371/JOURNAL.PNTD.0003917

Experimental method

X-RAY DIFFRACTION (1.3 Å)