4CFI

3D structure of FliC from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	287
Collection date	2013-07-10
Spacegroup name	P 21 21 21
Unit cell lengths	23.470, 72.780, 125.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.830 - 1.300
R-factor	0.13774
Rwork	0.136
R-free	0.16461
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3K8V 1IO1 AND 3V47
RMSD bond length	0.008
RMSD bond angle	1.172
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.260	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.050	0.280
Number of reflections	51380
<I/σ(I)>	14	4.8
Completeness [%]	99.7	99.3
Redundancy	4.3	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.1 M HEPES PH 7.5, 25% PEG 6000 AND 0.1 M LITHIUM CHLORIDE