4CFI
3D structure of FliC from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 287 |
| Collection date | 2013-07-10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.470, 72.780, 125.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.830 - 1.300 |
| R-factor | 0.13774 |
| Rwork | 0.136 |
| R-free | 0.16461 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3K8V 1IO1 AND 3V47 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.260 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 51380 | |
| <I/σ(I)> | 14 | 4.8 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 25% PEG 6000 AND 0.1 M LITHIUM CHLORIDE |
