4CCT

Dengue 1 cryo-EM reconstruction

Replaces:  4AZX

Summary for 4CCT

• Entry DOI: 10.2210/pdb4cct/pdb
• Related: 4B03
• EMDB information: 2141 2142
• Descriptor: DENGUE VIRUS 1 E PROTEIN, DENGUE VIRUS 1 M PROTEIN (2 entities in total)
• Functional Keywords: virus, flavivirus
• Biological source: DENGUE VIRUS 1; More
• Cellular location: Virion membrane ; Multi-pass membrane protein : G3F5K5 G3F5K5
• Total number of polymer chains: 6
• Total formula weight: 185853.67

Authors

Kostyuchenko, V.A.,Zhang, Q.,Tan, J.L.,Ng, T.S.,Lok, S.M. (deposition date: 2013-10-28, release date: 2013-11-06, Last modification date: 2024-05-08)

Primary citation

Kostyuchenko, V.A.,Zhang, Q.,Tan, J.L.,Ng, T.S.,Lok, S.M.
Immature and Mature Dengue Serotype 1 Virus Structures Provide Insight Into the Maturation Process.
J.Virol., 87:7700-, 2013

PubMed Abstract: Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-Å resolution, respectively. The subnanometer-resolution maps allow accurate placement of all of the surface proteins. Although the immature and mature viruses showed vastly different surface protein organizations, the envelope protein transmembrane (E-TM) regions remain in similar positions. The pivotal role of the E-TM regions leads to the identification of the start and end positions of all surface proteins during maturation.

PubMed: 23637416
DOI: 10.1128/JVI.00197-13

Experimental method

ELECTRON MICROSCOPY (4.5 Å)