EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 791 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Software | UCSF Chimera,EMAN |
| Symmetry | I |
