4BY9

The structure of the Box CD enzyme reveals regulation of rRNA methylation

Summary for 4BY9

• Entry DOI: 10.2210/pdb4by9/pdb
• NMR Information: BMRB: 19400
• Descriptor: SSR26, NOP5/NOP56 RELATED PROTEIN, 50S RIBOSOMAL PROTEIN L7AE, ... (5 entities in total)
• Functional Keywords: transferase-rna complex, rrna modification, transferase/rna
• Biological source: PYROCOCCUS FURIOSUS; More
• Cellular location: Cytoplasm : Q8U160
• Total number of polymer chains: 18
• Total formula weight: 385921.31

Authors

Lapinaite, A.,Simon, B.,Skjaerven, L.,Rakwalska-Bange, M.,Gabel, F.,Carlomagno, T. (deposition date: 2013-07-18, release date: 2013-10-09, Last modification date: 2024-06-19)

Primary citation

Lapinaite, A.,Simon, B.,Skjaerven, L.,Rakwalska-Bange, M.,Gabel, F.,Carlomagno, T.
The Structure of the Box C/D Enzyme Reveals Regulation of RNA Methylation.
Nature, 502:519-, 2013

PubMed Abstract: Post-transcriptional modifications are essential to the cell life cycle, as they affect both pre-ribosomal RNA processing and ribosome assembly. The box C/D ribonucleoprotein enzyme that methylates ribosomal RNA at the 2'-O-ribose uses a multitude of guide RNAs as templates for the recognition of rRNA target sites. Two methylation guide sequences are combined on each guide RNA, the significance of which has remained unclear. Here we use a powerful combination of NMR spectroscopy and small-angle neutron scattering to solve the structure of the 390 kDa archaeal RNP enzyme bound to substrate RNA. We show that the two methylation guide sequences are located in different environments in the complex and that the methylation of physiological substrates targeted by the same guide RNA occurs sequentially. This structure provides a means for differential control of methylation levels at the two sites and at the same time offers an unexpected regulatory mechanism for rRNA folding.

PubMed: 24121435
DOI: 10.1038/NATURE12581

Experimental method

SOLUTION NMR