4BY6

Yeast Not1-Not2-Not5 complex

Summary for 4BY6

• Entry DOI: 10.2210/pdb4by6/pdb
• Descriptor: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1, GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2, GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5, ... (8 entities in total)
• Functional Keywords: transcription, cterminal components of the ccr4_not complex
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Cytoplasm: P06100; Cytoplasm (Probable): Q12514
• Total number of polymer chains: 6
• Total formula weight: 236659.62

Authors

Bhaskar, V.,Basquin, J.,Conti, E. (deposition date: 2013-07-18, release date: 2013-10-16, Last modification date: 2024-05-08)

Primary citation

Bhaskar, V.,Roudko, V.,Basquin, J.,Sharma, K.,Urlaub, H.,Seraphin, B.,Conti, E.
Structure and RNA-Binding Properties of the not1-not2-not5 Module of the Yeast Ccr4-not Complex
Nat.Struct.Mol.Biol., 20:1281-, 2013

PubMed Abstract: The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-Å-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.

PubMed: 24121231
DOI: 10.1038/NSMB.2686

Experimental method

X-RAY DIFFRACTION (2.797 Å)