4BX8

Human Vps33A

Summary for 4BX8

• Entry DOI: 10.2210/pdb4bx8/pdb
• Related: 4BX9
• Descriptor: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33A, CHLORIDE ION (3 entities in total)
• Functional Keywords: protein transport, hops, membrane trafficking, sec1/munc18 proteins, tethering
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 2
• Total formula weight: 138503.61

Authors

Graham, S.C.,Wartosch, L.,Gray, S.R.,Scourfield, E.J.,Deane, J.E.,Luzio, J.P.,Owen, D.J. (deposition date: 2013-07-09, release date: 2013-07-24, Last modification date: 2024-05-08)

Primary citation

Graham, S.C.,Wartosch, L.,Gray, S.R.,Scourfield, E.J.,Deane, J.E.,Luzio, J.P.,Owen, D.J.
Structural Basis of Vps33A Recruitment to the Human Hops Complex by Vps16.
Proc.Natl.Acad.Sci.USA, 110:13345-, 2013

PubMed Abstract: The multisubunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for late endosome-lysosome and autophagosome-lysosome fusion in mammals. We have determined the crystal structure of the human HOPS subunit Vps33A, confirming its identity as a Sec1/Munc18 family member. We show that HOPS subunit Vps16 recruits Vps33A to the human HOPS complex and that residues 642-736 are necessary and sufficient for this interaction, and we present the crystal structure of Vps33A in complex with Vps16(642-736). Mutations at the binding interface disrupt the Vps33A-Vps16 interaction both in vitro and in cells, preventing recruitment of Vps33A to the HOPS complex. The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes.

PubMed: 23901104
DOI: 10.1073/PNAS.1307074110

Experimental method

X-RAY DIFFRACTION (2.4 Å)