4BWL

Structure of the Y137A mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate, N-acetyl-D-mannosamine and N- acetylneuraminic acid

Summary for 4BWL

• Entry DOI: 10.2210/pdb4bwl/pdb
• Related: 4BWK
• Descriptor: N-ACETYLNEURAMINATE LYASE, 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid, PENTAETHYLENE GLYCOL, ... (6 entities in total)
• Functional Keywords: lyase, directed evolution, substrate specificity, protein engineering
• Biological source: ESCHERICHIA COLI; More
• Cellular location: Cytoplasm: P0A6L4 P0A6L4
• Total number of polymer chains: 4
• Total formula weight: 135663.71

Authors

Campeotto, I.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Berry, A. (deposition date: 2013-07-03, release date: 2014-02-19, Last modification date: 2023-12-20)

• Primary citation: Daniels, A.D.,Campeotto, I.,Van Der Kamp, M.W.,Bolt, A.H.,Trinh, C.H.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Mulholland, A.J.,Berry, A.; The Reaction Mechanism of N-Acetylneuraminic Acid Lyase Revealed by a Combination of Crystallography, Qm/Mm Simulation and Mutagenesis.; Acs Chem.Biol., 9:1025-, 2014; PubMed: 24521460; DOI: 10.1021/CB500067Z

Experimental method

X-RAY DIFFRACTION (2 Å)