4BWL

Structure of the Y137A mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate, N-acetyl-D-mannosamine and N- acetylneuraminic acid

4BWL の概要

• エントリーDOI: 10.2210/pdb4bwl/pdb
• 関連するPDBエントリー: 4BWK
• 分子名称: N-ACETYLNEURAMINATE LYASE, 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid, PENTAETHYLENE GLYCOL, ... (6 entities in total)
• 機能のキーワード: lyase, directed evolution, substrate specificity, protein engineering
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Cytoplasm: P0A6L4 P0A6L4
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 135663.71

構造登録者

Campeotto, I.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Berry, A. (登録日: 2013-07-03, 公開日: 2014-02-19, 最終更新日: 2023-12-20)

• 主引用文献: Daniels, A.D.,Campeotto, I.,Van Der Kamp, M.W.,Bolt, A.H.,Trinh, C.H.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Mulholland, A.J.,Berry, A.; The Reaction Mechanism of N-Acetylneuraminic Acid Lyase Revealed by a Combination of Crystallography, Qm/Mm Simulation and Mutagenesis.; Acs Chem.Biol., 9:1025-, 2014; PubMed: 24521460; DOI: 10.1021/CB500067Z

実験手法

X-RAY DIFFRACTION (2 Å)