4BW9

PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP

4BW9 の概要

• エントリーDOI: 10.2210/pdb4bw9/pdb
• 関連するPDBエントリー: 4BWA
• 分子名称: PYRROLYSINE--TRNA LIGASE, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (6 entities in total)
• 機能のキーワード: ligase
• 由来する生物種: METHANOSARCINA MAZEI
• 細胞内の位置: Cytoplasm (By similarity): Q8PWY1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34379.49

構造登録者

Schneider, S.,Vrabel, M.,Gattner, M.J.,Fluegel, V.,Lopez-Carillo, V.,Carell, T. (登録日: 2013-07-01, 公開日: 2013-07-31, 最終更新日: 2023-12-20)

主引用文献

Schneider, S.,Gattner, M.J.,Vrabel, M.,Flugel, V.,Lopez-Carrillo, V.,Prill, S.,Carell, T.
Structural Insights Into Incorporation of Norbornene Amino Acids for Click Modification of Proteins
Chem.Bio.Chem., 14:2114-, 2013

PubMed Abstract: Three for two: by using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.

PubMed: 24027216
DOI: 10.1002/CBIC.201300435

実験手法

X-RAY DIFFRACTION (2.35 Å)