4BRB
Crystal structure of the integral membrane enzyme DgkA-ref, delta 7
Summary for 4BRB
| Entry DOI | 10.2210/pdb4brb/pdb |
| Descriptor | DIACYLGLYCEROL KINASE, (2R)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE, ZINC ION, ... (6 entities in total) |
| Functional Keywords | transferase, lipid metabolism, membrane protein |
| Biological source | ESCHERICHIA COLI |
| Total number of polymer chains | 6 |
| Total formula weight | 88684.86 |
| Authors | Li, D.,Caffrey, M. (deposition date: 2013-06-04, release date: 2014-06-18, Last modification date: 2023-12-20) |
| Primary citation | Li, D.,Caffrey, M. Renaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic. Sci.Rep., 4:5806-, 2014 Cited by PubMed Abstract: Membrane proteins play vital roles in the life of the cell and are important therapeutic targets. Producing them in large quantities, pure and fully functional is a major challenge. Many promising projects end when intractable aggregates or precipitates form. Here we show how such unfolded aggregates can be solubilized and the solution mixed with lipid to spontaneously self-assemble a bicontinuous cubic mesophase into the bilayer of which the protein, in a confined, chaperonin-like environment, reconstitutes with 100% efficiency. The test protein, diacylglycerol kinase, reconstituted in the bilayer of the mesophase, was then crystallized in situ by the in meso or lipid cubic phase method providing an X-ray structure to a resolution of 2.55 Å. This highly efficient, inexpensive, simple and rapid approach should find application wherever properly folded, membrane reconstituted and functional proteins are required where the starting material is a denatured aggregate. PubMed: 25055873DOI: 10.1038/SREP05806 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
Download full validation report
