4BRB
Crystal structure of the integral membrane enzyme DgkA-ref, delta 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.500, 91.570, 143.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.233 - 2.550 |
R-factor | 0.2196 |
Rwork | 0.217 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.055 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.790 | 2.620 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.120 | 1.000 |
Number of reflections | 32610 | |
<I/σ(I)> | 7.9 | 2.2 |
Completeness [%] | 98.3 | 97.9 |
Redundancy | 5.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 0.06 M MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREES CELCIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |