4BQU

Japanin from Rhipicephalus appendiculatus bound to cholesterol: Orthorhombic crystal form

Summary for 4BQU

• Entry DOI: 10.2210/pdb4bqu/pdb
• Descriptor: JAPANIN, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• Functional Keywords: cholesterol-binding protein, tick
• Biological source: RHIPICEPHALUS APPENDICULATUS
• Total number of polymer chains: 2
• Total formula weight: 40282.91

Authors

Roversi, P.,Johnson, S.,Preston, S.,Austyn, J.M.,Nuttall, P.,Lea, S.M. (deposition date: 2013-06-02, release date: 2014-06-18, Last modification date: 2023-12-20)

Primary citation

Roversi, P.,Johnson, S.,Preston, S.G.,Nunn, M.A.,Paesen, G.C.,Austyn, J.M.,Nuttall, P.A.,Lea, S.M.
Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Sci Rep, 7:16057-16057, 2017

PubMed Abstract: Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives.

PubMed: 29167574
DOI: 10.1038/s41598-017-16413-2

Experimental method

X-RAY DIFFRACTION (2.36 Å)