4BH6

Insights into degron recognition by APC coactivators from the structure of an Acm1-Cdh1 complex

Summary for 4BH6

• Entry DOI: 10.2210/pdb4bh6/pdb
• Descriptor: APC/C ACTIVATOR PROTEIN CDH1, APC/C-CDH1 MODULATOR 1 (3 entities in total)
• Functional Keywords: anaphase promoting complex, acm1, cell cycle, ubiquitination, d box
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Cytoplasm: P53197
• Total number of polymer chains: 16
• Total formula weight: 339299.80

Authors

He, J.,Chao, W.C.H.,Zhang, Z.,Yang, J.,Cronin, N.,Barford, D. (deposition date: 2013-03-29, release date: 2013-06-05, Last modification date: 2024-10-23)

Primary citation

He, J.,Chao, W.C.,Zhang, Z.,Yang, J.,Cronin, N.,Barford, D.
Insights Into Degron Recognition by Apc/C Coactivators from the Structure of an Acm1-Cdh1 Complex.
Mol.Cell, 50:649-, 2013

PubMed Abstract: The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C regulatory proteins is not defined at the atomic level. Here, from the crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the molecular basis for D box recognition. Additional interactions between Acm1 and Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer coactivator-specific protein functions including substrate association. We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor.

PubMed: 23707760
DOI: 10.1016/J.MOLCEL.2013.04.024

Experimental method

X-RAY DIFFRACTION (2.9 Å)