4BH6
Insights into degron recognition by APC coactivators from the structure of an Acm1-Cdh1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.431, 188.142, 93.440 |
| Unit cell angles | 90.00, 92.13, 90.00 |
Refinement procedure
| Resolution | 69.270 - 2.900 |
| R-factor | 0.22075 |
| Rwork | 0.219 |
| R-free | 0.25725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aez |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.400 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 72799 | |
| <I/σ(I)> | 8 | 1.9 |
| Completeness [%] | 96.2 | 98 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PEG, pH 6 |
