4AY2

Capturing 5' tri-phosphorylated RNA duplex by RIG-I

4AY2 の概要

• エントリーDOI: 10.2210/pdb4ay2/pdb
• 関連するPDBエントリー: 2YKG
• 分子名称: PROBABLE ATP-DEPENDENT RNA HELICASE DDX58, 5'-R-PPP(GP*GP*CP*GP*CP*GP*GP*CP*UP*UP*CP*GP*GP*CP *CP*GP*CP*GP*CP*C)-3', ZINC ION, ... (6 entities in total)
• 機能のキーワード: hydrolase-rna complex, innate immunity, hydrolase/rna
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: O95786
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85784.49

構造登録者

Luo, D.,Pyle, A.M. (登録日: 2012-06-17, 公開日: 2012-10-03, 最終更新日: 2024-10-09)

主引用文献

Luo, D.,Kohlway, A.,Vela, A.,Pyle, A.M.
Visualizing the Determinants of Viral RNA Recognition by Innate Immune Sensor Rig-I.
Structure, 20:1983-, 2012

PubMed Abstract: Retinoic acid inducible gene-I (RIG-I) is a key intracellular immune receptor for pathogenic RNAs, particularly from RNA viruses. Here, we report the crystal structure of human RIG-I bound to a 5' triphosphorylated RNA hairpin and ADP nucleotide at 2.8 Å resolution. The RNA ligand contains all structural features that are essential for optimal recognition by RIG-I, as it mimics the panhandle-like signatures within the genome of negative-stranded RNA viruses. RIG-I adopts an intermediate, semiclosed conformation in this product state of ATP hydrolysis. The structure of this complex allows us to visualize the first steps in RIG-I recognition and activation upon viral infection.

PubMed: 23022350
DOI: 10.1016/J.STR.2012.08.029

実験手法

X-RAY DIFFRACTION (2.8 Å)