4AP2

Crystal structure of the human KLHL11-Cul3 complex at 2.8A resolution

4AP2 の概要

• エントリーDOI: 10.2210/pdb4ap2/pdb
• 分子名称: KELCH-LIKE PROTEIN 11, CULLIN-3, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: ubiquitination, e3 ligase, cell cycle
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: Q13618
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83130.71

構造登録者

Canning, P.,Cooper, C.D.O.,Krojer, T.,Filippakopoulos, P.,Ayinampudi, V.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,von Delft, F.,Bullock, A.N.,Structural Genomics Consortium (SGC) (登録日: 2012-03-30, 公開日: 2012-05-09, 最終更新日: 2024-05-08)

主引用文献

Canning, P.,Cooper, C.D.O.,Krojer, T.,Murray, J.W.,Pike, A.C.W.,Chaikuad, A.,Keates, T.,Thangaratnarajah, C.,Hojzan, V.,Marsden, B.D.,Gileadi, O.,Knapp, S.,von Delft, F.,Bullock, A.N.
Structural Basis for Cul3 Assembly with the Btb-Kelch Family of E3 Ubiquitin Ligases.
J.Biol.Chem., 288:7803-, 2013

PubMed Abstract: Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein "3-box" motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.

PubMed: 23349464
DOI: 10.1074/JBC.M112.437996

実験手法

X-RAY DIFFRACTION (2.8 Å)