4AMA

Crystal Structure of N-acetylneuraminic acid lyase from Staphylococcus aureus with the chemical modification thia-lysine at position 165 in complex with pyruvate

4AMA の概要

• エントリーDOI: 10.2210/pdb4ama/pdb
• 関連するPDBエントリー: 4AH7 4AHO 4AHP 4AHQ
• 分子名称: N-ACETYLNEURAMINATE LYASE (2 entities in total)
• 機能のキーワード: lyase, conversion on pyruvate and d-acetyl mannosamine to n-acetylneuraminic acid
• 由来する生物種: STAPHYLOCOCCUS AUREUS SUBSP. AUREUS NCTC 8325
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 135444.89

構造登録者

Timms, N.,Polyakova, A.,Windle, C.L.,Trinh, C.H.,Nelson, A.,Pearson, A.R.,Berry, A. (登録日: 2012-03-08, 公開日: 2013-01-23, 最終更新日: 2019-07-10)

• 主引用文献: Timms, N.,Windle, C.L.,Polyakova, A.,Ault, J.R.,Trinh, C.H.,Pearson, A.R.,Nelson, A.,Berry, A.; Structural insights into the recovery of aldolase activity in N-acetylneuraminic acid lyase by replacement of the catalytically active lysine with gamma-thialysine by using a chemical mutagenesis strategy.; Chembiochem, 14:474-481, 2013; PubMed: 23418011; DOI: 10.1002/cbic.201200714

実験手法

X-RAY DIFFRACTION (2.35 Å)