4AMA

Crystal Structure of N-acetylneuraminic acid lyase from Staphylococcus aureus with the chemical modification thia-lysine at position 165 in complex with pyruvate

Summary for 4AMA

Entry DOI	10.2210/pdb4ama/pdb
Related	4AH7 4AHO 4AHP 4AHQ
Descriptor	N-ACETYLNEURAMINATE LYASE (2 entities in total)
Functional Keywords	lyase, conversion on pyruvate and d-acetyl mannosamine to n-acetylneuraminic acid
Biological source	STAPHYLOCOCCUS AUREUS SUBSP. AUREUS NCTC 8325
Total number of polymer chains	4
Total formula weight	135444.89
Authors	Timms, N.,Polyakova, A.,Windle, C.L.,Trinh, C.H.,Nelson, A.,Pearson, A.R.,Berry, A. (deposition date: 2012-03-08, release date: 2013-01-23, Last modification date: 2019-07-10)
Primary citation	Timms, N.,Windle, C.L.,Polyakova, A.,Ault, J.R.,Trinh, C.H.,Pearson, A.R.,Nelson, A.,Berry, A. Structural insights into the recovery of aldolase activity in N-acetylneuraminic acid lyase by replacement of the catalytically active lysine with gamma-thialysine by using a chemical mutagenesis strategy. Chembiochem, 14:474-481, 2013 Cited by PubMed: 23418011 DOI: 10.1002/cbic.201200714 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.35 Å)

Structure validation