4AC5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Summary for 4AC5

Related7PRC 1DXR 2WJM 1PRC 2X5V 1VRN 3PRC 6PRC 1R2C 2WJN 2X5U 5PRC 2PRC 2JBL
DescriptorPHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, REACTION CENTER PROTEIN H CHAIN, REACTION CENTER PROTEIN L CHAIN, ... (10 entities in total)
Functional Keywordsphotosynthesis, lipidic-sponge phase, reaction center, electron transport, cell membrane, metal- binding, transmembrane, formylation, chromophore, lipoprotein
Biological sourceBLASTOCHLORIS VIRIDIS
Total number of polymer chains4
Total molecular weight141823.99
Authors
Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,DePonte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R. (deposition date: 2011-12-14, release date: 2012-02-15, Last modification date: 2018-11-14)
Primary citation
Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,Deponte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R.
Lipidic Phase Membrane Protein Serial Femtosecond Crystallography.
Nat.Methods, 9:263-, 2012
PubMed: 22286383 (PDB entries with the same primary citation)
DOI: 10.1038/NMETH.1867
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (8.2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.3765 0.2% 1.0% 4.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4ac5
no rotation
Molmil generated image of 4ac5
rotated about x axis by 90°
Molmil generated image of 4ac5
rotated about y axis by 90°