4AC5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Summary for 4AC5

• Entry DOI: 10.2210/pdb4ac5/pdb
• Related: 1DXR 1PRC 1R2C 1VRN 2JBL 2PRC 2WJM 2WJN 2X5U 2X5V 3PRC 5PRC 6PRC 7PRC
• Descriptor: PHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, 15-cis-1,2-dihydroneurosporene, REACTION CENTER PROTEIN H CHAIN, ... (10 entities in total)
• Functional Keywords: photosynthesis, lipidic-sponge phase, reaction center, electron transport, cell membrane, metal- binding, transmembrane, formylation, chromophore, lipoprotein
• Biological source: BLASTOCHLORIS VIRIDIS; More
• Total number of polymer chains: 4
• Total formula weight: 141823.99

Authors

Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,DePonte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R. (deposition date: 2011-12-14, release date: 2012-02-15, Last modification date: 2024-11-06)

Primary citation

Johansson, L.C.,Arnlund, D.,White, T.A.,Katona, G.,Deponte, D.P.,Weierstall, U.,Doak, R.B.,Shoeman, R.L.,Lomb, L.,Malmerberg, E.,Davidsson, J.,Nass, K.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Bozek, J.D.,Caleman, C.,Coffee, R.,Coppola, N.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, R.,Hartmann, A.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kimmel, N.,Kirian, R.A.,Maia, F.R.N.C.,Marchesini, S.,Martin, A.V.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Sierra, R.,Soltau, H.,Starodub, D.,Stellato, F.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wahlgren, W.Y.,Wang, X.,Weidenspointner, G.,Wunderer, C.,Fromme, P.,Chapman, H.N.,Spence, J.C.H.,Neutze, R.
Lipidic Phase Membrane Protein Serial Femtosecond Crystallography.
Nat.Methods, 9:263-, 2012

PubMed Abstract: X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

PubMed: 22286383
DOI: 10.1038/NMETH.1867

Experimental method

X-RAY DIFFRACTION (8.2 Å)