4AC5
Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE AMO |
| Synchrotron site | SLAC LCLS |
| Beamline | AMO |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 6.2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.500, 84.600, 375.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.100 - 8.200 |
| R-factor | 0.35239 |
| Rwork | 0.351 |
| R-free | 0.38400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.650 |
| Data reduction software | CrystFEL (0.1.0 INDEXAMAJIG) |
| Data scaling software | MONTE (CARLO INTEGRATION) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 7.620 |
| High resolution limit [Å] | 7.400 | 7.370 |
| Rmerge | 0.500 | |
| Number of reflections | 2431 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 85.0 | 21.1 |
| Redundancy | 8 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | BATCH CRYSTALLIZATIONS WERE SET UP IN SEPTUM-SEALED GLASS VIALS CONTAINING 100 UL PROTEIN (20-30 MG/ML) , 100 UL LIPIDIC SPONGE PHASE (12 % MONOOLEIN, 17.5 % JEFFAMINE M- 600, 1.0 M HEPES PH 8.0, 0.7 M (NH4)2SO4, 2.5 % 1,2,3- HEPTANETRIOL) AND 50 UL 1.0-1.2 M TRI-SODIUM CITRATE |
