4ABO

Mal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubules

4ABO の概要

• エントリーDOI: 10.2210/pdb4abo/pdb
• 関連するPDBエントリー: 1FFX 1IA0 1JFF 1TUB
• EMDBエントリー: 2004 2005 2006
• 分子名称: TUBULIN BETA CHAIN, TUBULIN ALPHA-1A CHAIN, MICROTUBULE INTEGRITY PROTEIN MAL3, ... (5 entities in total)
• 機能のキーワード: structural protein, cytoskeleton, gtpase, end binding
• 由来する生物種: SCHIZOSACCHAROMYCES POMBE (FISSION YEAST); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton : Q10113
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 421064.68

構造登録者

Maurer, S.P.,Fourniol, F.J.,Bohner, G.,Moores, C.A.,Surrey, T. (登録日: 2011-12-09, 公開日: 2012-06-06, 最終更新日: 2024-05-08)

主引用文献

Maurer, S.P.,Fourniol, F.J.,Bohner, G.,Moores, C.A.,Surrey, T.
Ebs Recognize a Nucleotide-Dependent Structural CAP at Growing Microtubule Ends.
Cell(Cambridge,Mass.), 149:371-, 2012

PubMed Abstract: Growing microtubule ends serve as transient binding platforms for essential proteins that regulate microtubule dynamics and their interactions with cellular substructures. End-binding proteins (EBs) autonomously recognize an extended region at growing microtubule ends with unknown structural characteristics and then recruit other factors to the dynamic end structure. Using cryo-electron microscopy, subnanometer single-particle reconstruction, and fluorescence imaging, we present a pseudoatomic model of how the calponin homology (CH) domain of the fission yeast EB Mal3 binds to the end regions of growing microtubules. The Mal3 CH domain bridges protofilaments except at the microtubule seam. By binding close to the exchangeable GTP-binding site, the CH domain is ideally positioned to sense the microtubule's nucleotide state. The same microtubule-end region is also a stabilizing structural cap protecting the microtubule from depolymerization. This insight supports a common structural link between two important biological phenomena, microtubule dynamic instability and end tracking.

PubMed: 22500803
DOI: 10.1016/J.CELL.2012.02.049

実験手法

ELECTRON MICROSCOPY (8.6 Å)