4AB1
Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers
Summary for 4AB1
| Entry DOI | 10.2210/pdb4ab1/pdb |
| Related | 1MX1 1MX5 1MX9 1YA4 1YA8 1YAH 1YAJ |
| Descriptor | LIVER CARBOXYLESTERASE 1, 2-acetamido-2-deoxy-beta-D-glucopyranose, THIOCYANATE ION, ... (4 entities in total) |
| Functional Keywords | hydrolase, whole insect expression systems |
| Biological source | HOMO SAPIENS (HUMAN) |
| Total number of polymer chains | 1 |
| Total formula weight | 59067.68 |
| Authors | Greenblatt, H.M.,Otto, T.C.,Cerasoli, D.M.,Sussman, J.L. (deposition date: 2011-12-06, release date: 2012-03-07, Last modification date: 2024-10-23) |
| Primary citation | Greenblatt, H.M.,Otto, T.C.,Kirkpatrick, M.G.,Kovaleva, E.,Brown, S.,Buchman, G.,Cerasoli, D.M.,Sussman, J.L. Structure of Recombinant Human Carboxylesterase 1 Isolated from Whole Cabbage Looper Larvae. Acta Crystallogr.,Sect.F, 68:269-, 2012 Cited by PubMed Abstract: The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies. PubMed: 22442219DOI: 10.1107/S1744309112003326 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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