X-ray crystal structure of porcine insulin flash-cooled at high pressure

Summary for 4A7E

Related1ZEI 1B2G 3INS 1WAV 1B2B 1B2F 1M5A 9INS 1B2D 1DEI 1B17 1SDB 1B2A 1IZB 2TCI 1B2C 4INS 7INS 1IZA 3MTH 1B19 1MPJ 1ZNI 1B2E 1B18 4A7D
DescriptorINSULIN A CHAIN, INSULIN B CHAIN (3 entities in total)
Functional Keywordshormone, high-pressure cooling, high-pressure protein crystallography
Biological sourceSUS SCROFA (PIG)
Cellular locationSecreted P01315 P01315
Total number of polymer chains2
Total molecular weight5787.63
Burkhardt, A.,Warmer, M.,Panneerselvam, S.,Wagner, A.,Reimer, R.,Hohenberg, H.,Meents, A. (deposition date: 2011-11-14, release date: 2011-11-30, Last modification date: 2012-05-30)
Primary citation
Burkhardt, A.,Warmer, M.,Pannerselvam, S.,Wagner, A.,Zouni, A.,Reimer, R.,Hohenberg, H.,Meents, A.
Fast High-Pressure Freezing of Protein Crystals in Their Mother Liquor
Acta Crystallogr.,Sect.F, 68:495-, 2012
PubMed: 22505429 (PDB entries with the same primary citation)
DOI: 10.1107/S1744309112009670
MImport into Mendeley
Experimental method
NMR Information

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.222112.2%00MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4a7e
no rotation
Molmil generated image of 4a7e
rotated about x axis by 90°
Molmil generated image of 4a7e
rotated about y axis by 90°