1WAV

CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN

Summary for 1WAV

• Entry DOI: 10.2210/pdb1wav/pdb
• Descriptor: INSULIN, PHENOL, ZINC ION, ... (5 entities in total)
• Functional Keywords: hormone, insulin, phenol
• Biological source: Sus scrofa (pig); More
• Cellular location: Secreted: P01315 P01315
• Total number of polymer chains: 12
• Total formula weight: 35421.23

Authors

Liang, D.-C.,Ding, J.-H.,Chang, W.-R.,Wan, Z.-L. (deposition date: 1996-02-28, release date: 1997-02-28, Last modification date: 2024-10-30)

Primary citation

Ding, J.,Wan, Z.,Chang, W.,Liang, D.
Molecular replacement study on form-B monoclinic crystal of insulin.
Sci.China, Ser.C: Life Sci., 39:144-153, 1996

PubMed Abstract: The form-B monoclinic insulin crystal was obtained from the sodium citrate buffer with 1% zinc chloride, keeping phenolic content between 0.76% and 1.25%. Its space group is P2(1), cell constants are: a = 4.924 nm, b = 6.094 nm, c = 4.818 nm, beta = 95.8 degrees. There are 6 insulin molecules which form a hexamer. The initial phase was obtained by using rotation function program of X-PLOR program package and molecular packing program of our laboratory. The molecular model was chosen from 4 zinc bovine insulin hexamer. After the preliminary refinement by using the macromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemically restrained least-squared refinement on the difference Fourier maps. The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s. deviations from standard bond length and bond angle are 0.0022 nm and 4.7 degrees, respectively.

PubMed: 8760462

Experimental method

X-RAY DIFFRACTION (2.5 Å)