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4X3U

Crystal structure of chromobox homolog 7 (CBX7) chromodomain with Suramin

Summary for 4X3U
Entry DOI10.2210/pdb4x3u/pdb
Related4X3S 4X3T
DescriptorChromobox protein homolog 7, 8,8'-[CARBONYLBIS[IMINO-3,1-PHENYLENECARBONYLIMINO(4-METHYL-3,1-PHENYLENE)CARBONYLIMINO]]BIS-1,3,5-NAPHTHALENETRISULFON IC ACID (3 entities in total)
Functional Keywordscbx7, chromodomain, suramin, transcription
Biological sourceMus musculus (Mouse)
Cellular locationNucleus : Q8VDS3
Total number of polymer chains2
Total formula weight18056.31
Authors
Ren, C.,Zhou, M.M. (deposition date: 2014-12-01, release date: 2015-03-04, Last modification date: 2024-02-28)
Primary citationRen, C.,Morohashi, K.,Plotnikov, A.N.,Jakoncic, J.,Smith, S.G.,Li, J.,Zeng, L.,Rodriguez, Y.,Stojanoff, V.,Walsh, M.,Zhou, M.M.
Small-Molecule Modulators of Methyl-Lysine Binding for the CBX7 Chromodomain.
Chem.Biol., 22:161-168, 2015
Cited by
PubMed Abstract: Chromobox homolog 7 (CBX7) plays an important role in gene transcription in a wide array of cellular processes, ranging from stem cell self-renewal and differentiation to tumor progression. CBX7 functions through its N-terminal chromodomain (ChD), which recognizes trimethylated lysine 27 of histone 3 (H3K27me3), a conserved epigenetic mark that signifies gene transcriptional repression. In this study, we report the discovery of small molecules that inhibit CBX7ChD binding to H3K27me3. Our crystal structures reveal the binding modes of these molecules that compete against H3K27me3 binding through interactions with key residues in the methyl-lysine binding pocket of CBX7ChD. We further show that a lead compound, MS37452, derepresses transcription of Polycomb repressive complex target gene p16/CDKN2A by displacing CBX7 binding to the INK4A/ARF locus in prostate cancer cells. These small molecules have the potential to be developed into high-potency chemical modulators that target CBX7 functions in gene transcription in different disease pathways.
PubMed: 25660273
DOI: 10.1016/j.chembiol.2014.11.021
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.63 Å)
Structure validation

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