4X3U

Crystal structure of chromobox homolog 7 (CBX7) chromodomain with Suramin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	58.700, 58.700, 89.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.680 - 1.630
R-factor	0.16373
Rwork	0.160
R-free	0.23220
RMSD bond length	0.028
RMSD bond angle	3.318
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	22.680
High resolution limit [Å]	1.630
Number of reflections	20256
<I/σ(I)>	48.2
Completeness [%]	98.8
Redundancy	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	290	0.2 M lithium sulfate, 0.1 M Bis-Tris pH5.5, 25% PEG 3350