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4S0N

Crystal Structure of HLTF HIRAN Domain bound to DNA

Summary for 4S0N
Entry DOI10.2210/pdb4s0n/pdb
DescriptorHelicase-like transcription factor, 5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3', THYMIDINE-5'-MONOPHOSPHATE, ... (6 entities in total)
Functional Keywordsalpha+beta, dna 3'-end binding, dna binding protein-dna complex, dna binding protein/dna
Biological sourceHomo sapiens (human)
More
Cellular locationCytoplasm : Q14527
Total number of polymer chains8
Total formula weight69641.26
Authors
Chavez, D.A.,Eichman, B.F. (deposition date: 2015-01-02, release date: 2015-05-27, Last modification date: 2024-02-28)
Primary citationKile, A.C.,Chavez, D.A.,Bacal, J.,Eldirany, S.,Korzhnev, D.M.,Bezsonova, I.,Eichman, B.F.,Cimprich, K.A.
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Mol.Cell, 58:1090-1100, 2015
Cited by
PubMed Abstract: Stalled replication forks are a critical problem for the cell because they can lead to complex genome rearrangements that underlie cell death and disease. Processes such as DNA damage tolerance and replication fork reversal protect stalled forks from these events. A central mediator of these DNA damage responses in humans is the Rad5-related DNA translocase, HLTF. Here, we present biochemical and structural evidence that the HIRAN domain, an ancient and conserved domain found in HLTF and other DNA processing proteins, is a modified oligonucleotide/oligosaccharide (OB) fold that binds to 3' ssDNA ends. We demonstrate that the HIRAN domain promotes HLTF-dependent fork reversal in vitro through its interaction with 3' ssDNA ends found at forks. Finally, we show that HLTF restrains replication fork progression in cells in a HIRAN-dependent manner. These findings establish a mechanism of HLTF-mediated fork reversal and provide insight into the requirement for distinct fork remodeling activities in the cell.
PubMed: 26051180
DOI: 10.1016/j.molcel.2015.05.013
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.501 Å)
Structure validation

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