4QH3

X-ray structure of the adduct formed between bovine pancreatic ribonuclease and trans-dimethylamine methylamine dichlorido platinum(II)

Summary for 4QH3

• Entry DOI: 10.2210/pdb4qh3/pdb
• Related: 4QGZ
• Descriptor: Ribonuclease pancreatic, bis(chloranyl)-(dimethylamino)-(methylamino)platinum (3 entities in total)
• Functional Keywords: hydrolase, rna binding
• Biological source: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• Cellular location: Secreted: P61823
• Total number of polymer chains: 2
• Total formula weight: 30137.52

Authors

Russo Krauss, I.,Merlino, A. (deposition date: 2014-05-26, release date: 2014-07-30, Last modification date: 2024-11-20)

Primary citation

Messori, L.,Marzo, T.,Michelucci, E.,Russo Krauss, I.,Navarro-Ranninger, C.,Quiroga, A.G.,Merlino, A.
Interactions between Anticancer trans-Platinum Compounds and Proteins: Crystal Structures and ESI-MS Spectra of Two Protein Adducts of trans-(Dimethylamino)(methylamino)dichloridoplatinum(II).
Inorg.Chem., 53:7806-7808, 2014

PubMed Abstract: The adducts formed between trans-(dimethylamino)(methylamino)dichloridoplatinum(II), [t-PtCl2(dma)(ma)], and two model proteins, i.e., hen egg white lysozyme and bovine pancreatic ribonuclease, were independently characterized by X-ray crystallography and electrospray ionization mass spectrometry. In these adducts, the Pt(II) center, upon chloride release, coordinates either to histidine or aspartic acid residues while both alkylamino ligands remain bound to the metal. Comparison with the cisplatin derivatives of the same proteins highlights for [t-PtCl2(dma)(ma)] a kind of biomolecular metalation remarkably different from that of cisplatin.

PubMed: 25025479
DOI: 10.1021/ic5012583

Experimental method

X-RAY DIFFRACTION (2 Å)