4QH3
X-ray structure of the adduct formed between bovine pancreatic ribonuclease and trans-dimethylamine methylamine dichlorido platinum(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-16 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.824, 32.537, 67.237 |
Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
Resolution | 67.090 - 2.000 |
R-factor | 0.21729 |
Rwork | 0.214 |
R-free | 0.27507 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.900 |
High resolution limit [Å] | 2.000 |
Number of reflections | 14538 |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 20% PEG4000, 10 mM sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |