4NC3

Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.

Summary for 4NC3

• Entry DOI: 10.2210/pdb4nc3/pdb
• Related: 4IB4
• Descriptor: Chimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562, PALMITIC ACID, Ergotamine, ... (10 entities in total)
• Functional Keywords: serial femtosecond crystallography, human 5ht2b receptor, ergotamine, novel protein engineering, gpcr network, membrane protein, lipidic cubic phase, psi-biology, free electron laser, structural genomics, gpcr, membrane, signaling protein, electron transport
• Biological source: Homo sapiens (Human); More
• Cellular location: Cell membrane ; Multi- pass membrane protein : P41595
• Total number of polymer chains: 1
• Total formula weight: 53259.85

Authors

Liu, W.,Wacker, D.,Gati, C.,Han, G.W.,James, D.,Wang, D.,Nelson, G.,Weierstall, U.,Katritch, V.,Barty, A.,Zatsepin, N.A.,Li, D.,Messerschmidt, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Seibert, M.M.,Wang, C.,Shah, S.T.A.,Basu, S.,Fromme, R.,Kupitz, C.,Rendek, K.N.,Grotjohann, I.,Fromme, P.,Kirian, R.A.,Beyerlein, K.R.,White, T.A.,Chapman, H.N.,Caffrey, M.,Spence, J.C.H.,Stevens, R.C.,Cherezov, V.,GPCR Network (GPCR) (deposition date: 2013-10-23, release date: 2013-12-18, Last modification date: 2023-09-20)

• Primary citation: Liu, W.,Wacker, D.,Gati, C.,Han, G.W.,James, D.,Wang, D.,Nelson, G.,Weierstall, U.,Katritch, V.,Barty, A.,Zatsepin, N.A.,Li, D.,Messerschmidt, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Seibert, M.M.,Wang, C.,Shah, S.T.,Basu, S.,Fromme, R.,Kupitz, C.,Rendek, K.N.,Grotjohann, I.,Fromme, P.,Kirian, R.A.,Beyerlein, K.R.,White, T.A.,Chapman, H.N.,Caffrey, M.,Spence, J.C.,Stevens, R.C.,Cherezov, V.; Serial femtosecond crystallography of G protein-coupled receptors.; Science, 342:1521-1524, 2013; PubMed: 24357322; DOI: 10.1126/science.1244142

Experimental method

X-RAY DIFFRACTION (2.8 Å)