Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.

Summary for 4NC3

DescriptorChimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562, PALMITIC ACID, Ergotamine, ... (10 entities in total)
Functional Keywordsserial femtosecond crystallography, human 5ht2b receptor, ergotamine, novel protein engineering, gpcr network, membrane protein, lipidic cubic phase, psi-biology, free electron laser, structural genomics, gpcr, membrane, signaling protein, electron transport
Biological sourceHomo sapiens (Human)
Cellular locationCell membrane ; Multi- pass membrane protein  P41595
Total number of polymer chains1
Total molecular weight53259.85
Primary citation
Liu, W.,Wacker, D.,Gati, C.,Han, G.W.,James, D.,Wang, D.,Nelson, G.,Weierstall, U.,Katritch, V.,Barty, A.,Zatsepin, N.A.,Li, D.,Messerschmidt, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Seibert, M.M.,Wang, C.,Shah, S.T.,Basu, S.,Fromme, R.,Kupitz, C.,Rendek, K.N.,Grotjohann, I.,Fromme, P.,Kirian, R.A.,Beyerlein, K.R.,White, T.A.,Chapman, H.N.,Caffrey, M.,Spence, J.C.,Stevens, R.C.,Cherezov, V.
Serial femtosecond crystallography of G protein-coupled receptors.
Science, 342:1521-1524, 2013
PubMed: 24357322 (PDB entries with the same primary citation)
DOI: 10.1126/science.1244142
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.273301.0%76.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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