4C53

Crystal Structure of Guanarito virus GP2 in the post-fusion conformation

Summary for 4C53

• Entry DOI: 10.2210/pdb4c53/pdb
• Descriptor: PRE-GLYCOPROTEIN POLYPROTEIN GP COMPLEX, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: viral protein, arenavirus, venezuelan hemorrhagic fever, glycoprotein, fusion glycoprotein
• Biological source: GUANARITO VIRUS
• Total number of polymer chains: 3
• Total formula weight: 54236.04

Authors

Parsy, M.,Huiskonen, J.T.,Harlos, K.,Bowden, T.A. (deposition date: 2013-09-10, release date: 2013-10-02, Last modification date: 2023-12-20)

Primary citation

Parsy, M.,Harlos, K.,Huiskonen, J.T.,Bowden, T.A.
Crystal Structure of Venezuelan Hemorrhagic Fever Virus Fusion Glycoprotein Reveals a Class 1 Post-Fusion Architecture with Extensive Glycosylation.
J.Virol., 87:13070-, 2013

PubMed Abstract: Guanarito virus (GTOV) is an emergent and deadly pathogen. We present the crystal structure of the glycosylated GTOV fusion glycoprotein to 4.1-Å resolution in the postfusion conformation. Our structure reveals a classical six-helix bundle and presents direct verification that New World arenaviruses exhibit class I viral membrane fusion machinery. The structure provides visualization of an N-linked glycocalyx coat, and consideration of glycan dynamics reveals extensive coverage of the underlying protein surface, following virus-host membrane fusion.

PubMed: 24049182
DOI: 10.1128/JVI.02298-13

Experimental method

X-RAY DIFFRACTION (4.14 Å)