4C53
Crystal Structure of Guanarito virus GP2 in the post-fusion conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-02 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 |
Unit cell lengths | 98.500, 98.500, 78.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.440 - 4.140 |
R-factor | 0.256 |
Rwork | 0.255 |
R-free | 0.27580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mko |
RMSD bond length | 0.010 |
RMSD bond angle | 1.380 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.140 |
High resolution limit [Å] | 4.140 | 4.140 |
Rmerge | 0.090 | 1.000 |
Number of reflections | 5894 | |
<I/σ(I)> | 26.6 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.5 | 0.15 M LI2SO4, 0.1 M CITRIC ACID PH 3.5, 18% W/V PEG 6000 |