4AAD

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Summary for 4AAD

Entry DOI	10.2210/pdb4aad/pdb
Related	1AF5 1BP7 1G9Y 1G9Z 1MOW 1N3E 1N3F 1T9I 1T9J 1U0C 1U0D 2VBJ 2VBL 2VBN 2VBO 4AAB 4AAE 4AAF 4AAG
Descriptor	DNA ENDONUCLEASE I-CREI, 24MER DNA, GLYCEROL, ... (4 entities in total)
Functional Keywords	hydrolase-dna complex, gene targeting, protein-dna interaction, homing endonucleases, hydrolase/dna
Biological source	CHLAMYDOMONAS REINHARDTII More
Cellular location	Plastid, chloroplast: P05725
Total number of polymer chains	4
Total formula weight	50504.58
Authors	Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Epinat, J.C.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2011-12-01, release date: 2012-05-02, Last modification date: 2023-12-20)
Primary citation	Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Charles Epinat, J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40:6936-6945, 2012 Cited by PubMed: 22495931 DOI: 10.1093/NAR/GKS320 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation